BS ISO 14380:2011 BSl Standards Publication Water quality Determination of the acute toxicity to Thamnocephalus platyurus (Crustacea, Anostraca) bsi. ..making excellence a habit. BS ISO 14380:2011 BRITISH STANDARD National foreword This British Standard is the UK implementation of IS14380:2011. The UK participation in its preparation was entrusted to Technical Committee EH/3/5, Biological Methods. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. @BSI 2011 ISBN 9780 580 698736 ICS 13.060.70 Compliance with a British Standard cannot confer immunity from legal obligations. This British Standard was published under the authority of the Standards Policy and Strategy Committee on31December 2011 Amendments issued since publication Date Text affected BS ISO 14380:2011 INTERNATIONAL ISO STANDARD 14380 First edition 2011-11-15 Water quality Determination of the acute toxicity to Thamnocephalus platyurus (Crustacea, Anostraca) Qualitede I'eauDetermination dela toxiciteaigue envers Thamnocephalusplatyurus(Crustacea,Anostraca) Reference number ISO 14380:2011(E) ISO @ISO2011 BSISO14380:2011 ISO14380:2011(E) COPYRIGHTPROTECTEDDOCUMENT ISO 2011 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Casepostale 56 : CH-1211Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland ii @ ISO 2011 -All rights reserved BSISO14380:2011 ISO14380:2011(E) Contents Page Foreword .iv Introduction 1 Scope 2 Normativereferences 3 Terms and definitions 4 Principle 5 Test environment.. 6 Reagents,testorganisms and media. 2 7 Apparatus and materials. 3 8 Treatmentandpreparationofsamples 8.1 Special precautions. 8.2 Preparationofthestocksolutionsofsubstancestobetested 9 Procedure. 9.1 Hatching of the cysts. 9.2 Selectionoftestconcentrations 9.3 Preparationof the test and control solutions 9.4 Introduction of the organisms 9.5 Incubation ofthetestsystem. 9.6 Measurements 10 Estimation of the LC50 11 Referencetest 8 12 Validity criteria 13 Test report AnnexA (informative)Rapid test for determination of sublethal effects onThamnocephalusplatyurus (1 h exposure). .10 AnnexB (informative)Culturing ofThamnocephalusplatyurusforcystproduction. 16 Annex C (informative)Precision data. 19 Bibliography 20 @ ISO 2011-All rights reserved ili BS ISO14380:2011 ISO14380:2011(E) Foreword Iso (the International Organization for Standardization)is a worldwide federation of national standards bodies non-governmental, in liaison with isO, also take part in the work. IsO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. methods. iv ISO 2011 -All rights reserved BS ISO 14380:2011 ISO 14380:2011(E) Introduction The evaluation of harmful effects on water quality has for several years involved the performance of biological The test specified in this International Standard involves determination of the lethal effects on the fresh water to determine sublethal effects after a very short exposure time (1 h). a) this anostracan crustacean has a sensitivity to chemicals which is